Comprehensive Evaluation of the Embryotoxicity and Ototoxicity of a Car Diffuser on Zebrafish Larvae: Impacts on Morphology and Hair Cells.

Abstract

Diffusers release various chemical compounds into the environment, some of which may have ototoxic effects. Despite their widespread use, the safety profiles of these compounds remain largely unexplored. This study evaluated the potential toxicity of a car diffuser extract, Diff3, on zebrafish larvae by assessing morphology, survival rate, hair cell integrity, and rheotactic behavior. Forty wild-type zebrafish larvae at 4 h post-fertilization (hpf) were exposed to Diff3 and observed at 3 and 5 d